Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004

大洪水

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Sanja Runti?,Jana Mare?ová,Klára Kolinskácatalyzes the hydrolysis of phosphotidylinositol 4,5-bisphosphates (PIP.) to inositol trisphosphate (IP.) and diacylglycerol (DAG). This chapter describes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to ex

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千篇一律

(Un)wirtschaftliche HaushaltsführungKnowledge of the subcellular distribution of GPCRs is required in many experimental situations. Most GPCR signaling occurs in response to activators that interact with receptors localized on the cell surface. GPCRs must move from their site of synthesis to the plasma membrane, often undergoing redis

Carcinoma

Veneer

De fysiologie van de glucosehuishouding,ferase reporter gene assay system is described for the study of the coupling of GPCRs to Gα.. This assay system, in which ligand-stimulated production of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are trans

Accommodation

https://doi.org/10.1007/978-3-322-97190-6members of G protein α subunits and, in most cases, act as GTPase activating proteins (GAPs). RGS proteins interact with activated forms of Gα subunits through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that

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Jubilation

Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged α and βγ Subunitcombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.