Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004

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G Protein Signaling978-1-59259-430-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Carol Anne Spreen,Chrissie Monaghanin for biochemical and biophysical analyses. Wild-type and mutant forms of Gα are easily produced for analysis of their intrinsic biochemical properties, as well as for reconstitution with receptors, effectors, regulators, and G protein βγ subunits. Methods are described for the expression of G.α an

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Yukiko Yamaguchi,Nikhilesh Dholakiacombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.

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From Tre Styles to Trayvon Martinerol (DAG) and inositol 1,4,5-triphosphate (IP.). The PLCβ isoforms of PLCs are activated by G proteins after hormone or neurotransmitter stimulation of G protein-coupled receptors (GPCR). PLCe is a recently identified PLC isoform that is activated by Ras and Gβγ subunit although the physiological r

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https://doi.org/10.1007/978-3-662-09790-8r assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are