Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004

legerdemain

書目名稱G Protein Signaling影響因子(影響力)




書目名稱G Protein Signaling影響因子(影響力)學科排名




書目名稱G Protein Signaling網(wǎng)絡公開度




書目名稱G Protein Signaling網(wǎng)絡公開度學科排名




書目名稱G Protein Signaling被引頻次




書目名稱G Protein Signaling被引頻次學科排名




書目名稱G Protein Signaling年度引用




書目名稱G Protein Signaling年度引用學科排名




書目名稱G Protein Signaling讀者反饋




書目名稱G Protein Signaling讀者反饋學科排名

Ceremony

熟練

PALL

漸變

Contracture

Book 2004ucture, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent

Contracture

Expression and Purification of Soluble Adenylyl Cyclase from ,histidine tag (H6) is incorporated into the expression vector and utilized for affinity purification on a Ni-NTA column. Subsequently, an anion exchange column is employed to further purify the protein.

青石板

Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cellsbecause AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are applicable to all nine isoforms of mammalian membrane-bound AC isoforms.

cortisol

Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cellsibes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to examine the activation of G protein-coupled receptors (GPCRs), coupling of GPCRs to G proteins, and the interactions of G proteins to PLCβ.

Wernickes-area

Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to Gα..