Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004

Lobotomy

Selective Inhibition of G Protein-Mediated Pathways Using RGS Domainss through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that are mediated by the interacting G proteins. The method is particularly useful to differentiate between G.-mediated and G.-mediated pathways.

舊式步槍

Carol Anne Spreen,Chrissie Monaghan)-tagged subunits using metal chelate chromatography. Modification of Gα with myristate can be recapitulated in . by expressing .-myristoyltransferase (NMT) with its G protein substrate. Protocols for the production and purification of myristoylated Gα are presented.

TIA742

From Tre Styles to Trayvon Martin)-tagged PLC β the purification involves two steps, affinity chromatography with Ni-NTA agarose followed by heparin Sepharose chromatography. 6-His-tagged PLCε can be purified in a single step with nickel nitrilotriacetic acid-agarose (Ni-NTA) affinity chromatography.

TOXIN

Incivility as a Tool for Social Change,tion of a phosphatidylalcohol, which is a specific and unique marker for PLD activity. This protocol is highly sensitive for the detection of PLD activity following the stimulation of intact cells, being a valuable method for studying the regulation of PLD activity in vivo.

凝結(jié)劑

欺騙世家

Fortify

冰雹

吸氣

Headstrong