Titlebook: Selective Autophagy; Methods and Protocol Ioannis P. Nezis Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

hieroglyphic

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Methods for Monitoring Mitophagy Using mt-Keima,rom this bimodal excitation, a ratiometric signal may be calculated to quantify mitophagy in live cells. This chapter describes procedures for measuring mitophagy by flow cytometry and live cell confocal microscopy with mt-Keima.

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Methods for Monitoring NCOA4-Mediated Ferritinophagy,g iron homeostasis, heme synthesis, mitochondrial respiratory function, and ferroptosis, an iron-dependent form of cell death. Targeting ferritinophagy has emerged as a potential anticancer therapeutic strategy. In this context, we provide a flowchart of the procedures and accompanying protocols for monitoring ferritinophagic flux.

Jargon

resilience

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Methods to Monitor Nucleophagy in Yeast, for maintaining nucleus homeostasis and regulating nucleus functions. In the budding yeast ., nucleophagy occurs in two different manners: one involves autophagosome formation for the sequestration and vacuolar transport of nucleus-derived vesicles (NDVs), and the other proceeds with the invaginati

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Synthetic Dimerization Approaches for In Vivo Studies in ,n, where, and how proteins interact, making it possible to study their functions, dynamics, and cellular consequences at a molecular level. These methods are versatile, reversible, and adaptable, allowing the dissection of complex cellular processes and the engineering of cellular functions. Here, w

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Assessing Neuronal Mitophagy During Development in the Nematode ,tive autophagic removal of mitochondria, stands out as a prominent mechanism for efficient mitochondrial turnover, which is crucial for proper neuronal development and function. Dysfunctional mitochondria and disrupted mitophagy pathways have been linked to a diverse array of neurological disorders.