Titlebook: Selective Autophagy; Methods and Protocol Ioannis P. Nezis Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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Methods for Monitoring Mitophagy Using mt-Keima,a mitochondrial matrix targeting sequence and has bimodal excitation with peaks at 440?nM in neutral environments and 586?nM in acidic environments. From this bimodal excitation, a ratiometric signal may be calculated to quantify mitophagy in live cells. This chapter describes procedures for measuri

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Method to Purify Phase-Separated p62 Bodies Using Fluorescence-Activated Particle Sorting,important roles in intracellular quality control and stress responses. However, little is known about their constituents. In this chapter, we describe a method for purifying p62 bodies using fluorescence-activated particle sorting. This method contributes to the identification of novel components of