Titlebook: Selective Autophagy; Methods and Protocol Ioannis P. Nezis Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

重要

書目名稱Selective Autophagy影響因子(影響力)




書目名稱Selective Autophagy影響因子(影響力)學(xué)科排名




書目名稱Selective Autophagy網(wǎng)絡(luò)公開度




書目名稱Selective Autophagy網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Selective Autophagy被引頻次




書目名稱Selective Autophagy被引頻次學(xué)科排名




書目名稱Selective Autophagy年度引用




書目名稱Selective Autophagy年度引用學(xué)科排名




書目名稱Selective Autophagy讀者反饋




書目名稱Selective Autophagy讀者反饋學(xué)科排名

Condescending

Synthetic Dimerization Approaches for In Vivo Studies in ,e describe two chemically induced dimerization systems in the model organism . Using the autophagy pathway as an example, we show how these approaches can be used to dissect molecular events in cells.

允許

Assays to Study Mitophagy in Plants,ial marker lines followed by image analysis, chemical inhibitor treatment, and plant phenotype studies. These methods can be used in combination to identify the putative mitophagy regulators and understand their functions in mitochondrial-related activities in plants.

積極詞匯

Fluorescence Microscopy and Immunoblotting for Mitophagy in Budding Yeast,pregulated under prolonged respiration or nutrient starvation, anchored on the surface of mitochondria, and activated to recruit the autophagy machinery for the formation of autophagosomes surrounding mitochondria. In this chapter, we provide protocols to assess Atg32-mediated mitophagy using fluorescence microscopy and immunoblotting.

新義

CRANK

Studying Selective Autophagy of Protein Aggregates Using Particles Induced by Multimerization (PIMs use of pH-sensitive fluorescent proteins, such as GFP or mKeima, the PIM assay allows for direct visualization of aggregate clearance in cells. Here, we describe a protocol for the use of the PIM assay to study aggrephagy in live and fixed cells.

Control-Group

High-Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay,dition, a case study on peptide interaction with human Atg8 proteins (LC3/GABARAPs) is described. Finally, data analysis and quality control of FP assays?are discussed for the proper calculation of . values for the measured compounds.

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1064-3745 ation advice from the experts.This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggr