Titlebook: Recombinant Protein Protocols; Detection and Isolat Rocky S. Tuan Book 1997 Springer Science+Business Media New York 1997

BRAVE

Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growthimmunoassays for highly sensitive detection of many analytes (.–.). CSPD chemiluminescent 1,2-dioxetane substrate (.) for alkaline phosphatase has been used with Sapphire-II enhancing reagent for enzyme-linked immunoassay detection of chloramphenicol acetyltransferase and human growth hormone report

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Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporternserted downstream from the . gene of ., which encodes maltose-binding protein (MBP), resulting in the expression of an MBP fusion protein (.,.). The method uses the strong “tat” promoter and the . translation initiation signals to give high-level expression of the cloned sequences (.,.), and a one-

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Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein Ae first described system allowing affinity purification of expressed gene products. To date, a multitude of proteins have been produced as fusions to the IgG-binding domains of staphylococcal protein A, in several different hosts such as gram-positive and gram-negative bacteria (.,.), yeast (.), CHO

Overdose

Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteinstant of the streptavidin-biotin complex is approx 10..; the binding of streptavidin to biotin is one of the strongest noncovalent interactions found in biological systems. The extremely tight and specific biotin-binding ability of streptavidin has made this protein a very powerful biological tool fo

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Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins of proteins and to characterize and clone interacting proteins (.,.). The yeast transcriptional activator protein GAL4 contains the sequences necessary for DNA-binding, dimerization and nuclear localization within its N-terminal 147 amino acids (.). A dimer of this fragment can bind specifically to

珍奇

Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoproteinphenomenon of MDR was first observed in the clinic in tumors of patients undergoing chemotherapy. Multidrug resistance was recognized as a major factor contributing to the failure of chemotherapeutic treatment of cancer (.). Multidrug resistance also occurs in cultured cells selected for resistance

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Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatographs the reduced number of steps required for purification. However, owing to the high avidity of antibodies for their respective antigens, it is often difficult to elute the recombinant protein from the immunoaffinity column without denaturing the antibody or protein antigen. If the antibody binding e

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Yeast GAL4 Two-Hybrid System The system offers several innovations for identifying interacting proteins over conventional biochemical methods such as coimmunoprecipitation and affinity copurification. The two-hybrid system is the first genetic and molecular approach to detect interacting proteins, and the assay is performed in

Foam-Cells

Alternative Yeast Two-Hybrid SystemsBD) fused P1 and activation-domain (AD) fused P2 by assaying transcriptional activation of one or more reporter genes containing cognate DNA binding site for the DBD (.), a number of groups have developed variants of this approach for purposes such as mapping interaction domains on proteins, screeni