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Titlebook: Recombinant Protein Protocols; Detection and Isolat Rocky S. Tuan Book 1997 Springer Science+Business Media New York 1997

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Book 1997of methodologies to enhance the detection and isolation of their gene product(s) of interest. A large number of molecular tags and labels and their usage are described, including enzymes, ligand-binding moieties, immunodetectable molecules, as well as methods to detect interactive proteins, and gene
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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/824103.jpg
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Detection of β-Galactosidase and β-Glucuronidase Using Chemiluminescent Reporter Gene Assaystivity with a variety of detection techniques including radioisotopic, colorimetric, luminescent, fluorescent, and immunoassay methods (.–.). Chemiluminescent assays have been very effective in detecting enzyme labels in direct enzyme activity assays, immunoassays, and nucleic acid hybridization assays.
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Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antvels of heterologous protein. The combination of cloning and selection speeds up the generation of permanent cell lines, increases the number of potential insertion events that can be screened, and reduces the tedium of sequentially cloning then analyzing the production levels of each clone.
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