Titlebook: Recombinant Protein Protocols; Detection and Isolat Rocky S. Tuan Book 1997 Springer Science+Business Media New York 1997

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Histochemical and Fluorochrome-Based Detection of β-Galactosidasestaining fixed specimens, and the other for staining having cells. Each of these techniques has unique advantages and limitations, but together they offer a wide range of possibilities for taking advantage of β-gal as a reporter enzyme in a variety of applications.

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Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growthn used with Sapphire-II enhancing reagent for enzyme-linked immunoassay detection of chloramphenicol acetyltransferase and human growth hormone reporter gene products. These chemiluminescent reporter gene assay systems provide nonradioactive, simple, sensitive detection methods, performed in microplate or tube luminometers.

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Reporter Systemsreporter system is and outline the many uses of genetic reporters. Furthermore, the currently available reporter genes and assays will be described in terms of their specific applications and limitations.

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Human Placental Alkaline Phosphatase as a Marker for Gene Expressionns. To achieve this, it is frequently convenient to place a marker (or surrogate) gene under the genetic control of the regulatory sequences of interest. Expression of the marker gene is then monitored by analyzing accumulation of the encoded protein and this, in turn, serves as a measure of gene ex

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Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cellsments in transfected cells. As a reporter of gene expression, SEAP differs from intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase in that SEAP is secreted from transfected cells. SEAP can thus be assayed using a small portion of the culture medium. The SE

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