Titlebook: In Vivo Cellular Imaging Using Fluorescent Proteins; Methods and Protocol Robert M. Hoffman Book 2012 Springer Science+Business Media New Y

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Subcellular Imaging In Vivo: The Next GFP Revolution,o been used to color code the phases of the cell cycle which can now be followed in vivo. This technology has furthered our understanding of the spread of cancer at the subcellular level. Fluorescent proteins thereby provide the basis for the new field of in vivo cell biology.

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1064-3745 tation advice from the experts.The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology.? What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. .In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and P

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High-Resolution In Vivo Imaging of Fluorescent Proteins Using Window Chamber Models,cquisition of high-quality images down to the cellular or subcellular scale, exploiting the diverse array of optical contrast mechanisms, while also maintaining the native microenvironment of the tissue of interest. This makes these techniques applicable to a wide array of problems in the biomedical sciences.

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In Vivo Imaging of the Developing Mouse Embryonic Vasculature,ual blood cells. Imaging approaches described in this chapter can be used to analyze cardiovascular defects in mutant animals and can provide insights into understanding how genetic signaling pathways and physiological inputs regulate development.

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In Vivo Imaging of Oligonucleotide Delivery,n subsequent in vivo studies, an appropriate animal model must be developed for a better evaluation of gene-silencing effects on tumors. How to evaluate the effect of siRNA and miRNA in an in vivo therapeutic model is also important. Bioluminescence imaging is an optical imaging method that can evaluate RNAi in vivo.

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Screening Reef Corals for Novel GFP-Type Fluorescent Proteins by Confocal Imaging, of spectral colors, brightness, monomeric or dimeric states, faster maturation states, and photoactivity. Confocal imaging, coupled with microspectral detection, provides a rapid technique for in vivo characterization of FPs with desirable spectral and photoactive properties.

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In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models, tumor growth, and metastasis is achieved in vivo without the need for contrast agents, invasive techniques, or expensive imaging equipment. Transplantation of RFP-expressing tumor fragments onto the pancreas of GFP- or cyan fluorescent protein (CFP)-expressing transgenic nude mice was used to facil

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