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Titlebook: In Vivo Cellular Imaging Using Fluorescent Proteins; Methods and Protocol Robert M. Hoffman Book 2012 Springer Science+Business Media New Y

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書(shū)目名稱In Vivo Cellular Imaging Using Fluorescent Proteins
副標(biāo)題Methods and Protocol
編輯Robert M. Hoffman
視頻videohttp://file.papertrans.cn/464/463044/463044.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step details essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: In Vivo Cellular Imaging Using Fluorescent Proteins; Methods and Protocol Robert M. Hoffman Book 2012 Springer Science+Business Media New Y
描述.The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology.? What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. .In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols. presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..?.Authoritative and easily accessible, .In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols. .is the first volume in the new field of in vivo c
出版日期Book 2012
關(guān)鍵詞3-dimensional in vivo imaging; intravital imaging; red fluorescent proteins; tumor angiogenesis; tumor c
版次1
doihttps://doi.org/10.1007/978-1-61779-797-2
isbn_softcover978-1-4939-6149-8
isbn_ebook978-1-61779-797-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2012
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The Use of Fluorescent Proteins for Intravital Imaging of Cancer Cell Invasion,on in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-fla
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In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus, label tumor cells in vivo, as they are designed to selectively replicate within such cells. To target cancer cells, there is a need for tissue- or cell-specific promoters that are expressed in diverse tumor types and are silent in normal cells. Telomerase activation is considered to be a critical s
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Real-Time Fluorescence Imaging of Abdominal, Pleural, and Lymphatic Metastases,ly engineered to specifically infect tumor cells as cancer therapy can be further modified to have a visible marker gene for cancer staging. In this .hapter, we describe such a herpes simplex virus (HSV) modified to be detected by fluorescence. Other viruses so designed can be similarly used in canc
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