Titlebook: In Vivo Cellular Imaging Using Fluorescent Proteins; Methods and Protocol Robert M. Hoffman Book 2012 Springer Science+Business Media New Y

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The Use of Fluorescent Proteins for Intravital Imaging of Cancer Cell Invasion,on in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-fla

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Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatices of tumor progression and micrometastasis formation have been difficult to analyze and have been hampered by the inability to identify small numbers of tumor cells against a background of many host cells. The intrinsic brightness of fluorescent proteins has been taken advantage of to develop a tec

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In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus, label tumor cells in vivo, as they are designed to selectively replicate within such cells. To target cancer cells, there is a need for tissue- or cell-specific promoters that are expressed in diverse tumor types and are silent in normal cells. Telomerase activation is considered to be a critical s

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Real-Time Fluorescence Imaging of Abdominal, Pleural, and Lymphatic Metastases,ly engineered to specifically infect tumor cells as cancer therapy can be further modified to have a visible marker gene for cancer staging. In this .hapter, we describe such a herpes simplex virus (HSV) modified to be detected by fluorescence. Other viruses so designed can be similarly used in canc