Titlebook: Homing Endonucleases; Methods and Protocol David R. Edgell Book 2014 Springer Science+Business Media New York 2014 Binding and cleavage spe

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A Yeast-Based Recombination Assay for Homing Endonuclease Activity,ite of the break. Such mechanisms can thus be used for all the applications covered today by the generic name of “genome engineering”: targeted sequence insertion, removal, or editing. However, before being able to address those applications, the engineering of HEs must be mastered so that any poten