Titlebook: Homing Endonucleases; Methods and Protocol David R. Edgell Book 2014 Springer Science+Business Media New York 2014 Binding and cleavage spe

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Sarah K. Baxter,Andrew M. Scharenberg,Abigail R. Lambertndischen Reisebriefen“ zu dem friedlichen und harmlosen Volk der Singhalesen. Schon seit seiner Jugendzeit hegte der berühmte Naturforscher den brennenben Wunsch, eine Tropenreise auszuführen. ?Der Wunsch, die Wunder der Tropennatur von Angesicht zu sehen, ist für jeden Naturforscher, der sich die E

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Stefan Pellenz,Raymond J. Monnat Jr.gebende Natur mir nicht mehr neu und unbekannt war, einen ganz anderen Reiz für mich Auch bedeutete der Einsatz an harter Arbeit, deren Lohn je nach den Verh?ltnissen das gro?e Los oder nur eine Niete werden konnte, unter allen Umst?nden die herrlichste Befreiung aus der langen Einf?rmigkeit. In bez

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Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers,echanisms that govern the function and evolution of these genetic oddities have been well documented over the past few decades at the genetic, biochemical, and structural levels. This wealth of information has led to the manipulation and reprogramming of the endonucleases and to their exploitation i

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Bioinformatic Identification of Homing Endonucleases and Their Target Sites,The public genomic sequence databases contain thousands of HEGs. This is a large and diverse arsenal of potential genome editing tools. To make use of this natural resource, one needs to identify candidate HEGs. Due to their special relationship with a host gene, it is also possible to predict their

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,Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE,uclease promoters often lack recognizable promoters making predicting the transcriptional start site challenging. Furthermore, homing endonucleases are often expressed at very low levels making transcript mapping a challenge. Here, I present a 5′RLM-RACE protocol with special considerations for the

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PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydnce I-CreII is from a mobile . intron of ., it cleaves the . gene of an intronless-. strain of .. And it cleaves specifically in the . gene of ., which is naturally intronless. We have shown further that most of the repair products of an I-CreII-induced break in chloroplast DNA can be defined by PCR

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