Titlebook: Homing Endonucleases; Methods and Protocol David R. Edgell Book 2014 Springer Science+Business Media New York 2014 Binding and cleavage spe

背書(shū)

Book 2014 endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of

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PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes,lternative to synthesizing artificial endonucleases. Here, we describe methods for PCR-based screening of fungal mitochondrial rRNA genes for homing endonuclease encoding sequences, and we also provide protocols for the purification and biochemical characterization of putative native homing endonucleases.

Carcinogen

,Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE,e often expressed at very low levels making transcript mapping a challenge. Here, I present a 5′RLM-RACE protocol with special considerations for the expected abundance of homing endonucleases and for their potential to be subjected to RNA processing events.

聯(lián)合

Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sen. In order to delineate the target site specificity of HEs and facilitate HE engineering, we have developed a method for comprehensive target site profiling of HEs cleavage specificity using partially randomized target site libraries and high-throughput DNA sequencing.

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Taegun Kwon,Obed W. Odom,Weihua Qiu,David L. Herrinisen.Menschliche N?he, Feiern.Wie gehen wir mit Globalisierung um?.Es ist kaum zu verhindern, dass aus einer begrenzten Epidemie erneut eine globale Pandemie wird..Was haben wir gelernt?.Was k?nnen und müssen wir zukünftig besser machen?.Corona zwingt uns zu ?nderungen. Das n?chste Virus 978-3-662-63555-1978-3-662-63556-8

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META

Mohamed Hafez,Tuhin Kumar Guha,Chen Shen,Jyothi Sethuraman,Georg Hausnernse electromagnetic radiation are of primary and utmost importance as many of those objects are accessible only by detection of the electromagnetic radiation they emit. As such effects are typical for more remote planetary and astrophysical objects, the present chapter attempts to generalize a few o