Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993

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Subcloning for DNA Sequencing, are very inefficient at completing the last few small sequence gaps that remain near the end of the project. Various nonrandom subcloning methods have also been developed, utilizing partial restriction digests (.), BAL-3 1 digestion (.), exonuclease III digestion (l0–13), and DNase I (.).