Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993

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Electrophoresis of Sequence Reaction Samples,single-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (.).

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David Muhr,Michael Affenzeller,Josef KüngHistorically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(.. This enzyme was derived from E. .. DNA Pol I which, in addition to polymerase activity, has both 3′–5′ and 5′–3′ exonuclease activities.

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Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1,Historically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(.. This enzyme was derived from E. .. DNA Pol I which, in addition to polymerase activity, has both 3′–5′ and 5′–3′ exonuclease activities.

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Panagiota Kotoula,Christos Makrisf DNA in . by cloning vehicles derived from M13mp or pUC made expensive physical separation techniques like ultracentrifugation unnecessary. Although today the polymerase chain reaction is a valuable alternative for the amplification of small DNA pieces (.), it cannot substitute for the construction

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https://doi.org/10.1007/978-3-319-92016-0e are based on the findings of Mandel and Higa (.) who demonstrated that incubation of cells with naked bacteriophage λ DNA in cold calcium chloride resulted in uptake of virus and that a transient heat-shock of the mixture greatly enhanced the efficiency of transfection. Subsequently, this method w

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