Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993

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K. Bennaceur,Z. Sahraoui,M. A. Nacersis of the labeled strands can easily be synthesized in automatic synthesizers or by hand, the latter of which is more laborious. Various so-called "universal sequencing" and "reverse sequencing" primers, complementary to the beginning of the polylinker region in M13 . cloning phages and plasmids, a

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Transfection of E. coli with M13 DNA,ficiency in some or all strains of . that have been tested. The mechanisms involved in DNA transformation of cells are not fully understood and even with the most efficient methods that are available the proportion of cells that become “competent” for transformation is limited to around 10% of the t