Titlebook: Calcium Signalling; Methods and Protocol Anna Raffaello,Denis Vecellio Reane Book 2019 Springer Science+Business Media, LLC, part of Spring

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220789.jpg

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https://doi.org/10.1007/978-1-4939-9018-4Ca2+; Organelle-Targeted Aequorin-Based Probes; ; Sensitive Dyes; ; FRET; Toxoplasma

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Aerodynamische Entwicklungswerkzeuge,elles in intact cells. After the binding of Ca. to three high-affinity binding sites, an irreversible reaction occurs leading to the emission of photons that is proportional to [Ca.]. While native aequorin is suitable for measuring cytosolic [Ca.] after cell stimulation in a range from 0.5 to 10?μM,