Titlebook: Calcium Signalling; Methods and Protocol Anna Raffaello,Denis Vecellio Reane Book 2019 Springer Science+Business Media, LLC, part of Spring

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In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle,n. However, microscopic observation of Ca. signalling in live skeletal muscle tissue has been hampered, in particular, by the combination of the high speed of Ca. transients and the contractile properties that are inherent to muscle. The present chapter describes methods to visualize Ca. signals dur

巧辦法

TRPML1-/TFEB-Dependent Regulation of Lysosomal Exocytosis,al exocytosis and clearance of lysosomal accumulation in various cellular models of lysosomal storage disorders (LSDs). Here, we described methods to determine TFEB activation and lysosomal exocytosis that may represent innovative tools to study lysosomal function and to develop novel therapeutic ap

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Scintigraphy

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Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2,ions. SOCE is mediated through the plasma membrane (PM) protein, Orai1, and the endoplasmic reticulum protein, stromal interaction molecule 1 (STIM1). One of the most well-established methods to study SOCE is using the Ca.-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-

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Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes,st abundant glial cells in the brain. Astrocytes respond to neurotransmitters with Ca. elevations which represent a key event in the modulation of local brain circuits played by these glial cells. Due to technical limitations, the study of Ca. signal dynamics in astrocytes has focused for decades al

男學(xué)院

Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes,. Reactive oxygen species (ROS) are important factors that influence these redox processes. Calcium ion (Ca.) dynamics and signals are also essential regulators of key cellular processes. Therefore, the combined and precise monitoring of ROS and Ca. in single cells, with a high spatial and temporal

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Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography ergetic effect of Ca. signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we d

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Anna Raffaello,Denis Vecellio ReaneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts