Titlebook: Calcium Signalling; Methods and Protocol Anna Raffaello,Denis Vecellio Reane Book 2019 Springer Science+Business Media, LLC, part of Spring

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In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in ,,ng and phototoxicity as possible to the sample. Light sheet fluorescence microscopy offers these capabilities, with the further chance to mount the sample in vertical position, mimicking the plant’s growth and physiological conditions..A protocol for plant preparation and mounting in a light sheet m

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Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibers by urements are based on the use of genetically encoded probes. Addition of targeting DNA sequences, in frame with the probe encoding sequence, ensures protein expression in specific compartments. The use of probes with different excitation spectra allows the simultaneous determination of cytosolic and

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