Titlebook: Viral Vectors for Gene Therapy; Methods and Protocol Curtis A. Machida Book 2003 Humana Press 2003

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1543-1894 iruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Chapter cont- butions provide perspective in the use of viral vectors for applications in the brain and in the central nervous sy

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Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous Sys before describing the methodology in detail, it is important to briefly consider the structure and life cycle of HSV1 and the modifications that must be made to the virus in order to generate an efficient gene delivery vector.

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Strategies to Adapt Adenoviral Vectors for Targeted Delivery,fficient gene expression in the affected tissue, while minimizing toxicity and expression in other tissues. The use of recombinant adenoviruses as vectors for gene therapy is restricted by the widespread distribution of the coxsackie and adenovirus receptor (CAR) (.,.), which allows infection of a r

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Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain,mal models of human disease and in several human phase I clinical trials (.–.). Adenoviruses are easily purified to the high titers required for in vivo administration and they are efficient in transducing terminally differentiated cells such as neurons and glial cells, resulting in high levels of t