Titlebook: Viral Vectors for Gene Therapy; Methods and Protocol Curtis A. Machida Book 2003 Humana Press 2003

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Packaging Cell Lines for Generating Replication-Defective and Gutted Adenoviral Vectors,r genome that encodes numerous overlapping open reading frames (.). Because wild-type Ad is infectious to humans, vectors used in the laboratory generally contain a deletion of one or more genes to limit their growth and replication on nonpermissive cells. One drawback to Ad vectors is that infectio

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Targeted Integration by Adeno-Associated Virus,ne therapy applications. Expression of foreign genes in eukaryotic cells is highly dependent upon the efficiency of integration events and the site of insertion. Integration can sometimes have detrimental effects on the host cell, such as insertional mutagenesis or activation of proto-oncogenes. For

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Development and Optimization of Adeno-Associated Virus Vector Transfer into the Central Nervous Syslization of the potential of gene therapy for neurological disorders and functional genomic analysis in the brain remains a significant challenge and is largely limited by current technology. Efficient gene transfer to neurons remains a bottleneck for widespread molecular genetic studies of the nerv

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A Method for Helper Virus-Free Production of Adeno-Associated Virus Vectors,adeno-associated virus (AAV), as a gene delivery vehicle. AAV vectors have been used to deliver genes to a wide variety of mammalian cells in culture, as well as to brain (.), retina (.,.), cochlea (.), skeletal muscle (.,.), cardiac muscle (.), liver (.), lung (.,.), central nervous system (.), and

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Novel Tools for Production and Purification of Recombinant Adeno-Associated Viral Vectors, with a rAAV plasmid vector (pAAV) and a helper/packaging plasmid, followed by over-infection with a helper virus, normally an adenovirus (Ad) at low multiplicity of infection (MOI) (.). The pAAV vector contains the transgene of interest flanked by the AAV inverted terminal repeats (ITRs) and the he

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