Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 20041st edition Humana Press 2004

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An In Vitro Transcription System for Studying Vaccinia Virus Late Genes,This chapter describes a protocol that allows accurate in vitro transcription of vaccinia virus late genes. In this method, extracts are made from vaccinia virus-infected cells and used as enzyme sources to produce mRNAs from plasmid templates containing late gene promoter sequences.

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Stuart N. IsaacsIncludes supplementary material:

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