Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 20041st edition Humana Press 2004

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Mammal

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情感脆弱

Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactfectious virus can be produced if purified poxvirus DNA is transfected into cells previously infected with another poxvirus. This process is termed . if the infecting virus is different from the transfected virus. We describe a method in which the high-frequency recombination and replication reactio

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Growing Poxviruses and Determining Virus Titer,gs. The main principle of isolating the virus is to mechanically lyse infected cells. The virus can then be semipurified by centrifugation through a sucrose cushion. Further purification can be achieved with a sucrose gradient. This chapter describes methods for the preparation of large-scale growth

Rodent

ULCER

Orthopoxvirus Diagnostics, amplification, with either restriction cleavage or sequencing of amplicons, has been gaining credibility as a more rapid, specific, sensitive, and often cost-saving technique for research and diagnostic laboratories. This chapter is consolidated using prior research papers from our laboratories wit

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