Titlebook: Vaccinia Virus; Methods and Protocol Jason Mercer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR.Viral

Vo2-Max

Visualizing Poxvirus Replication and Recombination Using Live-Cell Imaging,d replication of vaccinia virus DNA, and to also detect the products of poxvirus-catalyzed recombination reactions. We describe how to use the bacteriophage lambda DNA-binding protein, cro, as a way of labeling DNA within a cell when it is conjugated to fluorescent proteins. When used in conjunction

Constrain

Beatriz Perdiguero,Carmen Elena Gómez,Mariano Esteban

FLASK

John P. Shannon,Olena Kamenyeva,Glennys V. Reynoso,Heather D. Hickman

DEMN

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hypnotic

易發(fā)怒

High-Content Analyses of Vaccinia Plaque Formation,t subtle virus-host interactions and their impact on virus spread during multiple rounds of infection. These include virus infectivity, mode of virus spread, virus replication rate, and spatiotemporal spread efficacy. How this underlying information can be harnessed using a high-content imaging setup is discussed here.

火光在搖曳

Bioluminescence Imaging as a Tool for Poxvirus Biology,the detection of viral infection sites and the quantification of viral replication in the context of tissues of a living animal. The goal of this chapter is to provide a technical background for the evaluation of poxvirus infection in cells and animals through bioluminescence imaging technology using luciferase-expressing recombinant poxviruses.

Pantry

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Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins,iral–fluorescent fusion proteins by fluorescence microscopy is noninvasive and can be used to describe protein localization in live cells and track the intracellular movement of virus particles. This chapter describes a number of approaches for the construction of plasmids and subsequent generation and isolation of fluorescent recombinant viruses.