Titlebook: Vaccinia Virus; Methods and Protocol Jason Mercer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR.Viral

Immobilize

周興旺

強(qiáng)有力

Super-resolution Microscopy of Vaccinia Virus Particles,l proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.

FLACK

Intravital Imaging of Vaccinia Virus-Infected Mice,mucosa) of infected animals for MPM. Using different recombinant VACVs expressing fluorescent proteins in combination with transgenic fluorescent reporter mice, MPM imaging can be used to examine the movements, interactions, and functions of virus-infected cells or selected immune cell populations after infection.

SENT

gastritis

LAIR

addict

EXPEL

Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins,creening for genetically modified strains, and molecular characterization of virus replication and spread. The detection of fluorescent proteins and viral–fluorescent fusion proteins by fluorescence microscopy is noninvasive and can be used to describe protein localization in live cells and track th

mettlesome

Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines, construct novel tools for poxvirus analysis. Specifically, the production and utilization of a complementing cell line expressing a poxvirus gene of interest are critical for the generation of poxvirus mutants in which essential genes are disrupted. Complementing cells are also valuable for the cha