Titlebook: Urothelial Carcinoma; Methods and Protocol Michèle J. Hoffmann,Nadine T. Gaisa,Thorsten H. Ec Book 2023Latest edition The Editor(s) (if app

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Predictive Biomarkers of Response to Neoadjuvant Therapy in Muscle Invasive Bladder Cancerf bladder cancer have shown the potential role for DNA damage repair (DDR) gene alterations and molecular subtypes to guide therapy, but these need validation from prospective clinical trials. This chapter reviews candidate predictive biomarkers of response to neoadjuvant therapy in muscle invasive bladder cancer.

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Scoring Systems for Immunohistochemistry in Urothelial Carcinomaroscopic evaluation and scoring approaches for immunohistochemical stainings. We focus on commonly used and generally feasible approaches for different cellular compartments and comment on their utility in diagnostics and research practice.

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Characterization of Native COMPASS Complex in Urothelial Carcinoma Cells by Size Exclusion Chromatogons were then separated by 3–8% Tris–acetate gradient polyacrylamide gel and the COMPASS complex subunits KMT2C, UTX, WDR5, and RBBP5 were detected by immunoblotting. In this fashion, the formation of a COMPASS complex could be observed in UC cells with wild-type but not in cells with mutant KMT2C and KMTD.

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Tissue Slice Culture and Analysis of Tumor-Associated Hyaluronan in Urothelial Carcinomang of the complex mechanisms of hyaluronan metabolism in cancer, it has been proposed to use precision-cut tissue slice cultures prepared using freshly excised cancer tissue. Here we describe the protocol for establishing tissue slice cultures and analysis of tumor-associated hyaluronan in human urothelial carcinoma.

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Assessment of PD-L1 Status in Urothelial Cancerystectomy. Several challenges which are highlighted in this chapter affect PD-L1 testing in daily routine including availability of representative tissue materials, inter-observer variability, and different available PD-L1 immunohistochemistry assays with different analytical properties.

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Methods in Molecular Biologyhttp://image.papertrans.cn/u/image/944364.jpg

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https://doi.org/10.1007/978-1-0716-3291-8Molecular characterization; Targeted therapy; Carcinogenesis; Bladder microbiome; Personalized medicine