Titlebook: Unconventional Protein Secretion; Methods and Protocol Andrea Pompa,Francesca De Marchis Book 2016 Springer Science+Business Media New York

granite

Chemical Secretory Pathway Modulation in Plant Protoplasts-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence.

地殼

Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lce regulator (CFTR), constitutes the most common of rare diseases and certainly a paradigmatic one..Here we focus on five different approaches that allow biochemical and cellular characterization of CFTR from its co-translational insertion into the ER membrane to its delivery to the plasma membrane.

手術(shù)刀

Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantificationisolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.

悲痛

FANG

Role and Characterization of Synuclein-γ Unconventional Protein Secretion in Cancer Cellss malignant phenotypes of cancer cells. Here, we describe methods for the characterization of SNCG as an unconventional secretion protein from cancer cells and investigation of the effect of extracellular SNCG on the phenotypes of cancer cells.

micturition