Titlebook: Unconventional Protein Secretion; Methods and Protocol Andrea Pompa,Francesca De Marchis Book 2016 Springer Science+Business Media New York

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Book 2016icular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organism

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Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Proteinrradiation as well as its siRNA- and chemical-mediated inhibition. In contrast to inflammasome activation of professional immune cells, UVB-irradiated HPKs represent a robust and physiological cell culture system for the analysis of UPS induced by active caspase-1.

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Analysis of Yeast Extracellular Vesiclesc approaches for analysis of EV lipids, microscopy techniques for analysis of both intracellular and extracellular vesicular compartments, interaction of EVs with host cells, and physical chemical analysis of EVs by dynamic light scattering.

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Following the Time-Course of Post-pollination Events by Transmission Electron Microscopy (TEM): Builibe a detailed protocol using the Transmission Electron Microscope to document the rapid cellular changes that occur in the stigmatic papillae in response to compatible versus self-incompatible pollen, at the pollen–stigma interface.