Titlebook: Synthetic mRNA; Production, Introduc Robert E. Rhoads Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 gene the

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Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cellsstics of mRNA. Antigen expression level and duration can be increased by multiple optimizations of an antigen-encoding mRNA template. Here, we describe different molecular modifications to a WT1-encoding mRNA construct in order to increase antigen expression and the subsequent introduction of mRNA into DCs.

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GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Useand viability. In order to comply with the directives and guidelines defined by the regulatory authorities, we report here a standardized and reproducible method for the manufacturing of clinical-grade mRNA-transfected DC.

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Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequencesse in regenerative medicine therapies, and engineering the genome by making specific alterations in DNA. This introductory chapter provides background information relevant to the following 20 chapters of this volume that present protocols for these applications of synthetic mRNA.

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Infant

Transfecting Human Monocytes with RNAon System. We further demonstrate that electroporation of monocytes with RNA represents a robust and highly relevant approach to modify monocytes for cell-based therapies. Last, the procedure described can readily be adapted to monocytes from different species, hence facilitating research in animal models.

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Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cance preparation. Here, we described a method of: (a) preparing and delivering synthetic FOXP3 mRNA into human DCs, (b) generating mature DCs, (c) generating FOXP3-specific cytotoxic T lymphocytes, and (d) evaluating cytotoxicity of FOXP3-specific cytotoxic T lymphocytes against inflammatory breast cancer cells.

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wo independent variables—culture and cultural heterogeneity—correctly identified more than eight out of ten states in terms of whether they did not or did ratify the ERA. The empirical indicator of culture was the state rate of labor force participation among married, husband-present women who had a

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t merely a descriptive language for financial risks (representational view of language) but a language that produces a “speech act” (pragmatic view of language) in the sense of financial models understood as speech acts: the financial . speaks and transforms the world through financial instruments a

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Robert E. Rhoadsgs, memories, traditions and identity contents. This symbolic conflict is, for us, an illustrative example of the importance of the role of names and their historicity in social psychology, in order to better understand the identity and memory processes they underlie and thus contribute to a more sy