Titlebook: Stable Isotope Probing; Methods and Protocol Marc G. Dumont,Marcela Hernández García Book 2019 Springer Science+Business Media, LLC, part o

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Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS,e isotopes (.C, .N, and .O) simultaneously. This method uses a high-density microarray to separate taxon-specific 16S (or 18S) rRNA genes and a high sensitivity magnetic sector secondary ion mass spectrometer (SIMS) to determine the relative isotope incorporation of the rRNA at each probe location.

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Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmen remain largely elusive because the vast majority of them are yet uncultured. Recent achievements in single cell stable isotope-labeled Raman spectroscopy enable direct investigation of function and activity of individual microbes in complex environmental communities. Here, this protocol describes a

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Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High-Resolution SIP,ibe the analysis of sequencing data using the multiple window high-resolution DNA-SIP method (MW-HR-SIP). MW-HR-SIP has improved accuracy over other methods and is easily implemented on the statistical platform R. We also discuss key experimental parameters to consider when designing DNA-SIP experim

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Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing, labeled with a heavy isotope..The laboratory protocol for qSIP is nearly identical to that for conventional stable isotope probing, with two key additions: (1) in qSIP, qPCR measurements are conducted on each density fraction recovered after isopycnic separation, and (2) in qSIP, multiple density f

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Stable Isotope Probing Techniques and Methodological Considerations Using 15N,le isotope probing was so far limited because of technical challenges related to the relative rarity of nitrogen in nucleic acids and proteins compared to carbon, and because of its absence in lipids. However, the recent adoption of high-throughput sequencing and statistical modelling methods to SIP

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