Titlebook: Stable Isotope Probing; Methods and Protocol Marc G. Dumont,Marcela Hernández García Book 2019 Springer Science+Business Media, LLC, part o

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Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing,ion of RNA-SIP with metatranscriptomic analysis enhances the detection and identification of mRNA from target microorganisms. In this chapter we provide a protocol for RNA-SIP, mRNA enrichment, and mRNA preparation for high-throughput sequencing using an example of targeting methanotrophs in rice field soil.

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DNA-Based Stable Isotope Probing,ten remain elusive. DNA-based stable isotope probing (DNA-SIP) provides a powerful means to establish a direct link between biogeochemical processes and the taxonomic identities of active microorganisms involved in the processes. Combined with high-throughput sequencing, it significantly aids in dec

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RNA Stable Isotope Probing (RNA-SIP),vironmental samples. Employing stable isotopes of common elements such as carbon and nitrogen, RNA-SIP exploits an increase in the buoyant density of RNA caused by the active metabolism and incorporation of heavier mass isotopes into the RNA after cellular utilization of labeled substrates pulsed in

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