Titlebook: Spermatogonial Stem Cells; Methods and Protocol Jon M. Oatley,Brian P. Hermann Book 2023 The Editor(s) (if applicable) and The Author(s), u

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Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells,undation of spermatogenesis and can either self-renew, producing more SSCs, or differentiate to initiate spermatogenesis and produce sperm. The biological potential of SSCs to produce and maintain spermatogenesis makes them a promising tool for the treatment of male infertility. However, translating

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,Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell—Part 1: Epigenomics Assays, plus computational methodology to integrate data from those four methodologies using Chromatin State Discovery and Characterization (ChromHMM) to obtain the most comprehensive overall assessment of epigenetic programming.

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Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells,togenesis in a transplant assay, there is no equivalent routine and accessible bioassay to test monkey and human SSCs or replicate their functions in vitro. This chapter describes progress characterizing, isolating, culturing, and transplanting SSCs in higher primates.

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Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia,workflows for the analysis of bulk RNA-seq from aggregate samples of hundreds to millions of cells and single-cell RNA-seq from individual cells. We also provide detailed protocols for using the R packages DESeq2 and Seurat, important parameters for successful implementation, and considerations for drawing conclusions from the results.

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Connor Cason,Tessa Lorded in many direc- tions and its techniques have intruded upon many areas of both fundamental and practical interest. The field itself has been enriched with many new phe- nomena and concepts that have further extended its scope and strengthened its connection with other areas. As a consequence, it i

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Vera van Melis,Lorena Roa-de la Cruz,Brian P. Hermannearch [1–2], to the generation of new frequencies [1], and most recently to all-optical signal processing [3]. In general, nonlinear optical interactions occur whenever the optical fields associated with one or more laser beams propagating in a material are large enough to produce polarization field