Titlebook: Spermatogonial Stem Cells; Methods and Protocol Jon M. Oatley,Brian P. Hermann Book 2023 The Editor(s) (if applicable) and The Author(s), u

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Primary Cultures of Spermatogonia and Testis Cells,o study the biology of SSCs as related to spermatogenesis, an in vitro, long-term expansion system of SSCs constitutes an ideal tool. In this chapter, we describe a robust culture system for mouse and rat SSCs in vitro. In the presence of GDNF, GFRα1, and bFGF, SSCs maintained on STO feeder layers w

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Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells,ant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would provide critical insight into the etiology of various reproductive conditions and disorders, including infertility..Recently, we reported the in vitro reconstitution of human prospermatogonial dev

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Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches,Much of our current understanding regarding the molecular mechanisms underlying SSC activity is derived from studies using conditional knockout mouse models. Here, we provide a guide for the selection and use of mouse strains to develop conditional knockout models for the study of SSCs, as well as t