Titlebook: Sorghum; Methods and Protocol Zuo-Yu Zhao,Jeff Dahlberg Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Striga

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書目名稱Sorghum影響因子(影響力)




書目名稱Sorghum影響因子(影響力)學(xué)科排名




書目名稱Sorghum網(wǎng)絡(luò)公開度




書目名稱Sorghum網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Sorghum被引頻次




書目名稱Sorghum被引頻次學(xué)科排名




書目名稱Sorghum年度引用




書目名稱Sorghum年度引用學(xué)科排名




書目名稱Sorghum讀者反饋




書目名稱Sorghum讀者反饋學(xué)科排名

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Zuo-Yu Zhao,Jeff DahlbergIncludes cutting-edge methods and protocols.provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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噱頭

primary method utilizes separation of the amino acids on a cation-exchange column, followed by postcolumn reaction with fluorescamine (.–.). This methodology will be presented in this article, but it will be placed in perspective with respect to the other approaches for amino acid analysis.

HOWL

o sequence low nanomolar to subnanomolar amounts of sample. The use of polybrene film to immobilize the sample was critical to the methodology, and was first introduced by Tarr (.). As demonstrated by Wittmann-Liebold (.), rigorous purification of reagents and solvents is also important for improved

橢圓

Larry Lambrightce for amino acid analysis has been classical ion-exchange chromatography followed by postcolumn derivatization with either ninhydrin (.–.) or a fluorogenic reagent (.–.). This method resolves most amino acids with good detection limits, especially if either .-phthaldialdehyde (OPA) or fluorescamine

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