Titlebook: Sorghum; Methods and Protocol Zuo-Yu Zhao,Jeff Dahlberg Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Striga

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書(shū)目名稱(chēng)Sorghum影響因子(影響力)




書(shū)目名稱(chēng)Sorghum影響因子(影響力)學(xué)科排名




書(shū)目名稱(chēng)Sorghum網(wǎng)絡(luò)公開(kāi)度




書(shū)目名稱(chēng)Sorghum網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書(shū)目名稱(chēng)Sorghum被引頻次




書(shū)目名稱(chēng)Sorghum被引頻次學(xué)科排名




書(shū)目名稱(chēng)Sorghum年度引用




書(shū)目名稱(chēng)Sorghum年度引用學(xué)科排名




書(shū)目名稱(chēng)Sorghum讀者反饋




書(shū)目名稱(chēng)Sorghum讀者反饋學(xué)科排名

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Zuo-Yu Zhao,Jeff DahlbergIncludes cutting-edge methods and protocols.provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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噱頭

primary method utilizes separation of the amino acids on a cation-exchange column, followed by postcolumn reaction with fluorescamine (.–.). This methodology will be presented in this article, but it will be placed in perspective with respect to the other approaches for amino acid analysis.

HOWL

o sequence low nanomolar to subnanomolar amounts of sample. The use of polybrene film to immobilize the sample was critical to the methodology, and was first introduced by Tarr (.). As demonstrated by Wittmann-Liebold (.), rigorous purification of reagents and solvents is also important for improved

橢圓

Larry Lambrightce for amino acid analysis has been classical ion-exchange chromatography followed by postcolumn derivatization with either ninhydrin (.–.) or a fluorogenic reagent (.–.). This method resolves most amino acids with good detection limits, especially if either .-phthaldialdehyde (OPA) or fluorescamine

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