Titlebook: Small Molecule Microarrays; Methods and Protocol Mahesh Uttamchandani,Shao Q. Yao Book 2017Latest edition Springer Science+Business Media N

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Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays,ease states. One such approach is the use of small molecule microarrays (SMMs). Typically, SMMs are generated by spatially arraying and covalently linking a library of small molecules to a glass surface. Next, incubation of the arrays with a fluorescently labeled RNA reveals binding interactions tha

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Profiling Phosphopeptide-Binding Domain Recognition Specificity Using Peptide Microarrays,ns are regulated by covalent posttranslational modifications such as phosphorylation and acetylation. These modifications can change the chemical nature of the interaction interfaces and modulate the binding affinity of the interacting partners. In signal transduction, the most frequent modification

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Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-Based Chemiluminescence Microegard to multiplex quantification, these tests can be performed as chemiluminescence microarray immunoassays, in which, in principle, the analyte in the sample and the same substance immobilized on the chip surface compete for a limited number of specific antibody binding sites. The amount of the sp

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Large-Scale Interaction Profiling of Protein Domains Through Proteomic Peptide-Phage Display Using lay (ProP-PD). In this chapter, we describe protocols for phage display for the identification of natural peptide binders for a given protein. We finally describe deep sequencing for the analysis of the proteomic peptide-phage display.

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Screening Mammalian Cells on a Hydrogel: Functionalized Small Molecule Microarray, preparation of mammalian cell-based microarrays using selectively captured of human prostate cancer cells (PC-3). This platform was then used in controlled drug release and measuring the associated drug effects on these cancer cells.

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