Titlebook: Small Molecule Microarrays; Methods and Protocol Mahesh Uttamchandani,Shao Q. Yao Book 2017Latest edition Springer Science+Business Media N

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The Expanding World of Small Molecule Microarrays,nal screening throughput and versatility. The concept at the heart of the technology is elegant, yet simple: by presenting large collections of molecules in high density on a flat surface, one is able to interrogate all possible interactions with desired targets, in just a single step. SMMs have bec

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Novel Substrates for Microarrays,nal substrates such as glass and nitrocellulose often entail complicated preparation processes such as the activation and functionalization of the reaction spots and surface blocking to prevent nonspecific molecule adsorption. In addition, coffee-ring morphology of the spots is a common issue in the

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Fabrication of Bio-function-Preserved Saccharide Microarray Chips with Cyanuric Chloride as a Rotatroblems, which have led to many strategies proposed but nearly none can immobilize small saccharides without losing their bio-affinity. Herein introduced is an easy strategy able to directly immobilize all intact saccharides on solid surface with excellent preservation of their molecular recognition

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Label-Free Sensing on Microarrays,f thousands of biomolecular interactions simultaneously, facilitating the functional characterization of these biomolecules in areas of genomics, proteomics, glycomics, and cytomics. Routinely, analysis of binding reactions between solution-phased probes and surface-immobilized targets involves some

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Optical Microscopy for Detecting Binding on Small Molecule Microarrays, simultaneous detection of up to thousands of biological interactions. Conventional detection technique employed in SMMs is fluorescence spectroscopy. However, the possibility of fluorescent labels affecting the binding properties of small molecules drives the development of label-free screening tec

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Array-on-Array Strategy For Activity-Based Enzyme Profiling,ts of spotting a first array with large droplets of enzymes with varying concentrations and subsequently spotting a second array with small droplets of fluorogenic substrate on top of the enzyme array. By varying the array on array spotting patterns of different classes of enzyme (e.g., proteases, p

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,Protein–Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays,action networks, to molecular fingerprinting and small molecular inhibitor discovery. The technique has significantly impacted both basic and applied research. The microarray platform can likewise enable high-throughput screening and discovery of protein–protein interaction (PPI) inhibitors. Herein