Titlebook: Retinal Degeneration; Methods and Protocol Bernhard H. F. Weber,Thomas Langmann Book 2019Latest edition Springer Science+Business Media, LL

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Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culturetal model systems that possess the same phagocytic machinery as RPE in situ. Upon experimental challenge with isolated photoreceptor outer segment fragments (POS), these cells promptly and efficiently recognize, bind, internalize, and digest POS. Here, we describe experimental procedures to isolate

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Porcine RPE/Choroidal Explant Culturesnment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4?h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the exp

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The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse E new possibilities for vision research: Organoids facilitate studies on retinal development and in vitro retinal disease modeling, as well as being valuable for drug testing. Further, retinal organoids also provide an unlimited cell source for cell replacement therapies. Here, we describe our protoc

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Light Damage Models of Retinal Degenerationover genetically determined degenerations is that light exposures can be manipulated according to the needs of the experimenter. Bright white light exposure can induce a synchronized burst of apoptosis in photoreceptors in a large retinal area which permits to study cellular and molecular events in

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Induction and Readout of Oxygen-Induced Retinopathy-established model to study normal as well as pathological angiogenic mechanisms in retina is the oxygen-induced retinopathy model in mice. This model is based on oxygen exposure of mouse pups during retinal vascular development, leading to a depletion of retinal capillaries. Upon return to room air

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Generation and Analysis of , Models of Retinal Degeneration Using CRISPR/Cas9n this system were limited by lack of a robust knockdown or knockout technology. Here we describe a protocol for generation of Cas9-edited . embryos. The technique introduces point mutations into the genome of . resulting in in-frame and out-of-frame insertions and deletions that allow modeling of b

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Gene Knockdown in Zebrafish (,) as a Tool to Model Photoreceptor Diseasesmple of this disease group is retinitis pigmentosa (RP), characterized by selective loss of photoreceptor cells. RP can be caused by dominant mutations in key factors of the pre-mRNA processing spliceosome. In these cases, the complex events leading to the RP phenotype can only insufficiently be ana