Titlebook: Retinal Degeneration; Methods and Protocol Bernhard H. F. Weber,Thomas Langmann Book 2019Latest edition Springer Science+Business Media, LL

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Monitoring Surface Reactions by Combined Western Blot-ELISA Analysisbined techniques of ELISA and immunoblotting, to determine complement activation and regulation on surfaces. The methods allow to follow part of the complement activation cascade and to determine the activity of complement regulators like factor H.

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CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivoular assays: site-specific PCR on P23H . cells treated with CRISPR/Cas9 and Western blotting analysis on retinal cells prepared from P23H . transgenic mice electroporated with CRISPR/Cas9 and GFP plasmids.

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978-1-4939-9360-4Springer Science+Business Media, LLC, part of Springer Nature 2019

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Bernhard H. F. Weber,Thomas LangmannIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/829203.jpg

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