Titlebook: Rab GTPases; Methods and Protocol Guangpu Li,Nava Segev Book 2021Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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Using GBP Nanotrap to Restore Autophagy in the Rab5 / Vps21 Mutant by Forcing Snf7 and Atg17 Interacalization in vivo. GFP binding protein (GBP) is a specific domain with high affinity to GFP. A novel technique with GBP fused protein X tagged with red fluorescence protein binding to GFP of GFP fused protein Y to establish a close association for proteins X and Y independently from other proteins

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Establishing Regulation of a Dynamic Process by Ypt/Rab GTPases : A Case for Cisternal Progression,ell microscopy in yeast cells since the Golgi cisternae are separated, as opposed to the stacked Golgi seen in other organisms. It is also possible to determine which trafficking machinery components are required for this process by studying mutants depleted for these components. However, determinin

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Qualitative and Quantitative Assessment of the Role of Endocytic Regulatory and/or Rab Proteins on years, new paradigms have uncovered roles for endocytic regulatory proteins in the control of mitochondrial fusion and fission, thus highlighting the utility of techniques for the study of mitochondrial morphology. Herein we detail methods to qualitatively and quantitatively measure the impact of se

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Book 2021Latest editiony-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Cutting-edge and comprehensive, .Rab GTPases: Methods and Protocols, Second Edition. is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond..

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