Titlebook: Osteoporosis and Osteoarthritis; Jennifer J. Westendorf,Andre J. van Wijnen Book 2015 Springer Science+Business Media. New York 2015 artic

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Identification of microRNAs in Human Plasmacharacterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.

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Immunohistochemistry of Skeletal Tissuesof interest, and then visualizing the bound antibody using a chromogen. Unlike in situ hybridization, which identifies gene transcripts in cells, IHC identifies the products themselves and provides information about their localization within cells (nuclear, cytoplasmic, or membrane) or extracellular

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In Vivo Axial Loading of the Mouse Tibia and enhance bone mass accrual in growing animals. These methods are also invaluable for understanding bone signaling pathways. Our focus here is on a particular loading model: in vivo axial compression of the mouse tibia. An advantage of loading the tibia is that changes are present in both the can

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