Titlebook: Osteoporosis and Osteoarthritis; Jennifer J. Westendorf,Andre J. van Wijnen Book 2015 Springer Science+Business Media. New York 2015 artic

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Induction of Fully Stabilized Cortical Bone Defects to Study Intramembranous Bone Regeneration of fracture healing, and in particular genetic mouse models, can be used to study the contributions of specific molecular switches to skeletal repair, as well as to recreate and exacerbate biological development and repair mechanisms in postnatal skeletons. Here, we describe methodology for produci

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Surgical Procedures and Experimental Outcomes of Closed Fractures in Rodent Modelsphenotypes and evaluate treatment modalities. Slight modifications in the fixation depth, to reduce surgical error associated with movement/dislocation of the k-wire fixation, were previously described. Here, we describe this method which involves the creation of a medial parapatellar incision, disl

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1064-3745 ation advice from the experts.Includes supplementary materia.In?.Osteoporosis and Osteoarthritis .expert investigators?detail?modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis.?New chapters describe?methods?to?genera

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Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cellsso provide detailed protocols for analyzing coculture pellets with cell tracking, proliferation assays, species specific polymerase chain reactions (PCR), short tandem repeats analysis, and histological examination.

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Generation of Induced Pluripotent Stem Cellsd in many laboratories around the world and the reader can initially follow these methods. However, not all cell types are easily transduced using viral vectors and other methods of delivering the reprogramming transcription factors may need to be tested.