Titlebook: Oligonucleotide Synthesis; Methods and Applicat Piet Herdewijn Book 2005 Humana Press 2005 DNA.PCR.gene expression.genes.siRNA

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Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms,reamlined production of biotin-labeled oligonucleotides. Protocols for the synthesis of biotinylated primers and purification of biotinylated sequencing products by means of streptavidin-coated magnetic beads are also presented.

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Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays,zation of the amplification reaction conditions using SYBR Green, (4) molecular beacon design, and (5) molecular beacon synthesis and characterization. The last section provides an example of a multiplex quantitative real-time PCR.

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1064-3745 eotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targ

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Book 2005l highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The appl

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Book 2005ications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective bindingof small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

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Dimethylthiarum Disulfide,ng DTD. This improvement allowed an overall 20% reduction in the solvent consumption and reduced the total synthesis time by 25%. The large-scale application of DTD has been successfully demonstrated by synthesis of therapeutically useful 20-mer phosphorothioate antisense oligonucleotides with excellent yield and purity.

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Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips, molecules bearing a bromomethyl- or aryldiazomethane-reactive group. In this chapter, we describe the preparation of the reactive label and protocols for efficient labeling of any nucleic acid sequence, DNA or RNA, prior to their hybridization, detection, and analysis on DNA chips.

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Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis,erns, of (deoxy)ribozyme kinetics, and of direct or competitive gel-shift assays. These methods can replace widely used radioisotope-based protocols, for example, for secondary structure mapping of RNA and for the characterization of nucleic acid ligand interactions.