Titlebook: Oligonucleotide Synthesis; Methods and Applicat Piet Herdewijn Book 2005 Humana Press 2005 DNA.PCR.gene expression.genes.siRNA

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High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing,hatic amine or hydroxide anion). We describe design, synthesis, and purification of fimers, and their use in protocols for direct sequencing of genomic deoxyribonucleic acid (DNA). Protocol for isolation of microbial genomic DNA is also reported.

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Nonenzymatic Template-Directed RNA Synthesis, chromatography or on gel electrophoresis with on-line detection applying a deoxyribonucleic acid sequencer. Three examples of primer extensions are given, one of which demonstrates how the reaction of several primers may be monitored simultaneously.

GIST

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Locked Nucleic Acid Synthesis,oved from the solid support as the 5′-end-.dimethoxytrityl (DMT) protected LNA oligomer by treatment with concentrated aqueous ammonia that also removes the phosphate and nucleobase protecting groups. The crude DMT protected LNA product can be purified using, for example, reversed-phase chromatography.

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Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides, inhibitor camptothecin to a triple helix-forming oligonucleotide is taken as an example of such synthesis. The described method has general interest because any functional ligand containing a primary or secondary amino group or aliphatic carboxyl group could be attached to the terminal phosphate of an oligonucleotide in a similar way.

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Methods in Molecular Biologyhttp://image.papertrans.cn/o/image/700862.jpg

CAB

https://doi.org/10.1385/1592598234DNA; PCR; gene expression; genes; siRNA

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Oligonucleotide Synthesis978-1-59259-823-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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