Titlebook: Notch Signaling Research; Methods and Protocol Dongyu Jia Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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FRET

Vartika Sharma,Nalani Sachan,Mousumi Mutsuddi,Ashim Mukherjee

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Manoj Panta,Andrew J. Kump,Kristopher R. Schwab,Shaad M. Ahmad

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Laura Márquez-Expósito,Carolina Lavoz,Elena Cantero-Navarro,Raúl R. Rodrigues-Diez,Sergio Mezzano,Ma

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Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases E EGF repeats using two different bacterial expression vectors, generated recombinant glycosyltransferases, and performed in vitro .-glycosylation and subsequent product analysis by mass spectrometry. The methods described here are useful for investigating the enzymatic activities of mutated glycosyl

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Debrief

Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context, spatiotemporal patterns of Notch-dependent transcriptional activation. These methods allow a powerful analysis of in vivo transcriptional activation and its dynamics in a whole tissue. Our methods can be adapted to essentially any tissue or cell type for any transcript.

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In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vasculdynamics in vivo and pulmonary arterial pressure ex vivo in mouse models of experimental PH. Using these experimental protocols, one can study the role of Notch or Notch signaling pathway in the pathogenic mechanisms of pulmonary vascular disease and develop novel therapies by targeting Notch ligand