Titlebook: New Technologies for Glutamate Interaction; Neurons and Glia Maria Kukley Book 2024 The Editor(s) (if applicable) and The Author(s), under

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0893-2336 ation advice from the experts.This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutam

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Surface Glutamate Receptor Nanoscale Organization with Super-Resolution Microscopy (dSTORM)ion of glutamate receptors. This approach is helping unveil the impact of this nanoscale organization on synaptic transmission and plasticity. In this chapter, we will describe the use of dSTORM to dissect the nanoscale organization of surface glutamate receptors and discuss sample preparation and analysis methods.

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Photooxidation of Genetically Encoded MiniSOG-Fused VGLUT2 for Identification of Glutamatergic Synapmbinant vesicular glutamate transporter-2 (VGLUT2) fused with mini singlet oxygen generator (miniSOG) and photooxidation of diaminobenzidine (DAB) to label glutamatergic SVs in nerve terminals in both fixed cultured cells and brain tissue.

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Computational Models of Astrocyte Function at Glutamatergic Synapsescted computational models of the involvement of astrocytes in glutamatergic transmission. The goal of this chapter is to present representative models of astrocyte function in conjunction with the biological questions they can investigate.

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Synaptic Integration at Neuron-OPC Synapsesion in OPCs. As neuronal activity-induced downstream signaling often occurs locally within the thin processes of OPCs, we will emphasize the methods to explore how OPCs integrate synaptic inputs within local compartments of the thin processes.

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0893-2336 urons and Glia. is a valuable resource for both neuronal and glial physiologists who are interested in learning about and developing new methods to further understand neuronal and neuron-glia synaptic transmission..978-1-0716-3744-9978-1-0716-3742-5Series ISSN 0893-2336 Series E-ISSN 1940-6045