Titlebook: Nanotoxicity; Methods and Protocol Qunwei Zhang Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Nanotoxicology

ostracize

nocturnal

Evaluation of Nrf2 with Exposure to Nanoparticles,verse transcription-polymerase chain reaction (RT-PCR), western blotting, immunofluorescence staining, electrophoretic mobility shift assay, DNase I footprinting, dimethylsulfate footprinting, protein pulse-chase analysis, and tert-butylhydroquinone treatment.

ADJ

Book 2019 to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nano

NOVA

Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies,2AX as a marker to assess the potential genotoxic effects of some nanoparticles in vitro and in vivo. In this chapter, we describe several useful methods for γ-H2AX detection, which can be used to evaluate the potential genotoxic effects of nanoparticles.

帶傷害

Quantification of Mitochondrial Genome Transcription in Male Reproductive Cells by Real-Time PCR,ochondrial function and can therefore be used as a quantitative index for evaluating nanotoxicity. This study describes how to use real-time PCR to evaluate the effects of nanomaterials on mitochondrial genome transcription in male reproductive cells.

irradicable

NUDGE

Pulmonary Function Testing in Animals, using the forced oscillation technique. Measurements of baseline lung mechanics are conducted in anesthetized, mechanically ventilated mice, followed by repeated measurements subsequent to inhalation challenge with aerosolized methacholine. General guidelines for data analysis are provided, and sample results are presented.

致敬

adequate-intake

Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and Higterials. Bacterial metabolome is extracted and metabolic fingerprint is recorded by .H-NMR. Using metabolomic databases, the tentative metabolites in the samples are revealed, which are further confirmed by UHPLC-HRMS. Finally, conducting a pathway analysis, the metabolic network is revealed.

Reservation

Methods for Assessing Mast Cell Responses to Engineered Nanomaterial Exposure,, including the most widely utilized carbon and metal-based ENMs, has been previously demonstrated; and therefore, understanding direct ENM interaction with mast cells at the cellular and molecular level is of critical importance for the safe implementation of ENMs into consumer products.